Review



listerin rescue  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    Addgene inc listerin rescue
    Figure 1 Non-stop mRNA protein products are degraded by the RQC in a <t>Listerin-dependent</t> manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).
    Listerin Rescue, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/listerin rescue/product/Addgene inc
    Average 91 stars, based on 2 article reviews
    listerin rescue - by Bioz Stars, 2026-03
    91/100 stars

    Images

    1) Product Images from "Impaired ribosome-associated quality control of C9orf72 arginine-rich dipeptide-repeat proteins."

    Article Title: Impaired ribosome-associated quality control of C9orf72 arginine-rich dipeptide-repeat proteins.

    Journal: Brain : a journal of neurology

    doi: 10.1093/brain/awac479

    Figure 1 Non-stop mRNA protein products are degraded by the RQC in a Listerin-dependent manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).
    Figure Legend Snippet: Figure 1 Non-stop mRNA protein products are degraded by the RQC in a Listerin-dependent manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).

    Techniques Used: Construct, Expressing, Control, Fluorescence, Over Expression, Flow Cytometry

    Figure 5 NEMF is recruited to DPR inclusions. (A) Representative confocal images of colocalization of NEMF and R-rich DPR proteins (arrowheads) ex pressed in HEK 293T cells (scale bar = 10 µm). (B) Representative confocal images of colocalization of NEMF and R-rich EGFP-DPR reporters (arrowheads) in I3N neurons (scale bar = 5 µm). (C) Confocal images show colocalization of DPR inclusions (poly-GA and poly-GR) and NEMF in frontal cortex and cerebellar granular layer of C9orf72 human tissue (scale bar, 5 µm). (D) Representative double immunofluorescence confocal images of DPR inclusions (poly-GA and poly-GR) and RQC factors (listerin and VCP) in frontal cortex of C9orf72-mutation carriers (scale bar = 5 µm). (E) Quantification of propor tion of DPR inclusions (poly-GA and poly-GR) that co-localize with RQC complex factors NEMF, listerin and VCP in frontal cortex and cerebellum from five C9orf72-expansion cases (total number of inclusions counted for poly-GA n = 90–130 or n = 1049–1201 and for poly-GR n = 41–55 or n = 62–114 in front al cortex and cerebellum, respectively; frontal cortex: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0668 and VCP P = 0.6507; cerebellum: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0767 and VCP P = 0.1403).
    Figure Legend Snippet: Figure 5 NEMF is recruited to DPR inclusions. (A) Representative confocal images of colocalization of NEMF and R-rich DPR proteins (arrowheads) ex pressed in HEK 293T cells (scale bar = 10 µm). (B) Representative confocal images of colocalization of NEMF and R-rich EGFP-DPR reporters (arrowheads) in I3N neurons (scale bar = 5 µm). (C) Confocal images show colocalization of DPR inclusions (poly-GA and poly-GR) and NEMF in frontal cortex and cerebellar granular layer of C9orf72 human tissue (scale bar, 5 µm). (D) Representative double immunofluorescence confocal images of DPR inclusions (poly-GA and poly-GR) and RQC factors (listerin and VCP) in frontal cortex of C9orf72-mutation carriers (scale bar = 5 µm). (E) Quantification of propor tion of DPR inclusions (poly-GA and poly-GR) that co-localize with RQC complex factors NEMF, listerin and VCP in frontal cortex and cerebellum from five C9orf72-expansion cases (total number of inclusions counted for poly-GA n = 90–130 or n = 1049–1201 and for poly-GR n = 41–55 or n = 62–114 in front al cortex and cerebellum, respectively; frontal cortex: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0668 and VCP P = 0.6507; cerebellum: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0767 and VCP P = 0.1403).

    Techniques Used: Immunofluorescence, Mutagenesis



    Similar Products

    listerin rescue  (Addgene inc)
    91
    Addgene inc listerin rescue
    Figure 1 Non-stop mRNA protein products are degraded by the RQC in a <t>Listerin-dependent</t> manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).
    Listerin Rescue, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/listerin rescue/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    listerin rescue - by Bioz Stars, 2026-03
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1 Non-stop mRNA protein products are degraded by the RQC in a Listerin-dependent manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).

    Journal: Brain : a journal of neurology

    Article Title: Impaired ribosome-associated quality control of C9orf72 arginine-rich dipeptide-repeat proteins.

    doi: 10.1093/brain/awac479

    Figure Lengend Snippet: Figure 1 Non-stop mRNA protein products are degraded by the RQC in a Listerin-dependent manner in mammalian cells. (A) Schematic of stalling reporters and EGFP constructs. (B) GFP versus RFP expression profile of stalling reporter with poly-A sequences compared to a no insert control (left). Ratio of median fluorescence intensity of RFP to GFP shows that poly-A sequences induce stalling independent of listerin. Data are shown as mean ± standard error of the mean (SEM) with all data points (two-way ANOVA, n = 3, reporter P < 0.0001, LTN1 KO P = 0.9771, interaction P = 0.8548) (C) Confocal images of HEK 293T and LTN1 KO cells expressing EGFP-NS reporter show accumulation and reduced clearance of NS protein in LTN1 KO cells over time (scale bar = 50 µm). (D) Listerin overexpression in LTN1 KO cells significantly reduces levels of NS reporter protein in cells quantified by flow cytometry. Percentage is shown as mean ± SEM (mixed effects model, n = 3, Ltn1:HEK 293T P = 0.1246, Ltn1:LTN1 KO #1 P = 0.0001, Ltn1:LTN1 KO #2 P < 0.0001).

    Article Snippet: For listerin rescue experiments, the LTN1_pCSdest plasmid used was a gift from Roger Reeves (#53855, Addgene).52 Plasmids expressing VCP wildtype (WT) or dominant-negative mutant (D2) were previously described.53 For NEMF antibody validation experiments, a plasmid expressing C-terminally HA tagged NEMF synthesized by GenScript was used. pDsRed-monomer-N1 plasmid was used as a transfection control.

    Techniques: Construct, Expressing, Control, Fluorescence, Over Expression, Flow Cytometry

    Figure 5 NEMF is recruited to DPR inclusions. (A) Representative confocal images of colocalization of NEMF and R-rich DPR proteins (arrowheads) ex pressed in HEK 293T cells (scale bar = 10 µm). (B) Representative confocal images of colocalization of NEMF and R-rich EGFP-DPR reporters (arrowheads) in I3N neurons (scale bar = 5 µm). (C) Confocal images show colocalization of DPR inclusions (poly-GA and poly-GR) and NEMF in frontal cortex and cerebellar granular layer of C9orf72 human tissue (scale bar, 5 µm). (D) Representative double immunofluorescence confocal images of DPR inclusions (poly-GA and poly-GR) and RQC factors (listerin and VCP) in frontal cortex of C9orf72-mutation carriers (scale bar = 5 µm). (E) Quantification of propor tion of DPR inclusions (poly-GA and poly-GR) that co-localize with RQC complex factors NEMF, listerin and VCP in frontal cortex and cerebellum from five C9orf72-expansion cases (total number of inclusions counted for poly-GA n = 90–130 or n = 1049–1201 and for poly-GR n = 41–55 or n = 62–114 in front al cortex and cerebellum, respectively; frontal cortex: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0668 and VCP P = 0.6507; cerebellum: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0767 and VCP P = 0.1403).

    Journal: Brain : a journal of neurology

    Article Title: Impaired ribosome-associated quality control of C9orf72 arginine-rich dipeptide-repeat proteins.

    doi: 10.1093/brain/awac479

    Figure Lengend Snippet: Figure 5 NEMF is recruited to DPR inclusions. (A) Representative confocal images of colocalization of NEMF and R-rich DPR proteins (arrowheads) ex pressed in HEK 293T cells (scale bar = 10 µm). (B) Representative confocal images of colocalization of NEMF and R-rich EGFP-DPR reporters (arrowheads) in I3N neurons (scale bar = 5 µm). (C) Confocal images show colocalization of DPR inclusions (poly-GA and poly-GR) and NEMF in frontal cortex and cerebellar granular layer of C9orf72 human tissue (scale bar, 5 µm). (D) Representative double immunofluorescence confocal images of DPR inclusions (poly-GA and poly-GR) and RQC factors (listerin and VCP) in frontal cortex of C9orf72-mutation carriers (scale bar = 5 µm). (E) Quantification of propor tion of DPR inclusions (poly-GA and poly-GR) that co-localize with RQC complex factors NEMF, listerin and VCP in frontal cortex and cerebellum from five C9orf72-expansion cases (total number of inclusions counted for poly-GA n = 90–130 or n = 1049–1201 and for poly-GR n = 41–55 or n = 62–114 in front al cortex and cerebellum, respectively; frontal cortex: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0668 and VCP P = 0.6507; cerebellum: Fisher’s exact test, NEMF P < 0.0001, listerin P = 0.0767 and VCP P = 0.1403).

    Article Snippet: For listerin rescue experiments, the LTN1_pCSdest plasmid used was a gift from Roger Reeves (#53855, Addgene).52 Plasmids expressing VCP wildtype (WT) or dominant-negative mutant (D2) were previously described.53 For NEMF antibody validation experiments, a plasmid expressing C-terminally HA tagged NEMF synthesized by GenScript was used. pDsRed-monomer-N1 plasmid was used as a transfection control.

    Techniques: Immunofluorescence, Mutagenesis